Journal: Antimicrobial Agents and Chemotherapy

Article Title: Selective Inhibition of Hepatitis C Virus Infection by Hydroxyzine and Benztropine

doi: 10.1128/AAC.02619-14

Figure Lengend Snippet: Selected compounds inhibit early aspects of HCV infection. (A) Huh7 cells were inoculated at a MOI of 10 in the presence of perphenazine (5 μM), hydroxyzine (5 μM), benztropine (7.5 μM), or DMSO as a control. Samples of cells and supernatants were collected to determine infectivity titers as well as intracellular HCV RNA levels by titration and RT-qPCR, respectively, at the indicated time points. (B) Inhibition of HCVtcp infection. Huh-7 cells were inoculated with HCVtcp in the presence of perphenazine (5 μM), hydroxyzine (5 μM), and benztropine (7.5 μM), and samples of the cells were assayed for luciferase activity 48 h postinoculation. Data are shown as averages and standard deviations of the results of two independent experiments performed in triplicate (n = 6). (C) Treatment of Huh-7 cells electroporated with a subgenomic HCV RNA replicon bearing a luciferase gene with perphenazine (5 μM), hydroxyzine (5 μM), benztropine (7.5 μM), or DMSO and 2mAde (10 μM) as negative and positive controls, respectively. Data are shown as averages and standard deviations of the results of two independent experiments performed in duplicate (n = 4). The statistical significance of the differences with the control data set was determined using Student's t test (*, P < 0.05; **, P < 0.01).

Article Snippet: 2mAde (2′-c-methyladenosine) was purchased from BOC Sciences (Shirley, NY).

Techniques: Infection, Control, Titration, Quantitative RT-PCR, Inhibition, Luciferase, Activity Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Selective Inhibition of Hepatitis C Virus Infection by Hydroxyzine and Benztropine

doi: 10.1128/AAC.02619-14

Figure Lengend Snippet: Impact of the selected compounds on persistently infected cell cultures. Persistently infected cells were treated for 48 h with perphenazine (5 μM), hydroxyzine (5 μM), benztropine (7.5 μM), or DMSO and 2mAde (10 μM) as negative and positive controls, respectively. Samples of cells and supernatants were collected to determine infectivity titers as well as intracellular HCV RNA levels by titration and RT-qPCR, respectively. Viruses present in the supernatant were partially purified using microfiltration devices to eliminate residual compound present in the supernatant. Infectivity titers were determined in the purified material. Data are shown as averages and standard deviations of the results of two independent experiments performed in triplicate (n = 6). The statistical significance of the differences with the control data set was determined using Student's t test (*, P < 0.05; **, P < 0.01).

Article Snippet: 2mAde (2′-c-methyladenosine) was purchased from BOC Sciences (Shirley, NY).

Techniques: Infection, Titration, Quantitative RT-PCR, Purification, Control